Ahmad Khan Kharal: A Punjabi Hero of 1857 by Saeed Bhutta

By Saeed Bhutta

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A contemporary assessment of 1 of my supply functions commented at the ‘rediscovery of tropomyosin’. i used to be tempted to jot down again in my rebuttal to the reviewer that I didn’t appreciate it were misplaced. Uncharacteristic adulthood prevailed and that i resisted the temptation, yet i used to be struck by means of the underlying statement that examine at the str- ture and serve as of tropomyosin has been just a little invisible, rather when it comes to the cytoskeleton isoforms.

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The mouse and human _-Tm (TPM1) genes are about 27 kb and 29 kb respectively and show a similar structure (Fig. 4). 45 For a comparison with the human gene, see Chapter 16. `-Tropomyosin Gene (TPM2) The mammalian ` tropomyosin gene ranges from 8 kb in human (TPM2) to 9 kb in mouse (TPM2) and 10kb in rat (`-TM). The gene spans 11 exons and contains a single promoter, a unique mutually exclusive internal exon (6a:6b) and two distinct carboxyl termini (9a:9d) (Fig. 4). 48,49 An additional product has been detected in humans (Chapter 16).

Molecular cloning and expression of the full-length tropomyosin gene from Trichinella spiralis. J Helminthol 2003; 77(1):57-63. 14. Jenkins RE, Taylor MJ, Gilvary NJ et al. Tropomyosin implicated in host protective responses to microfilariae in onchocerciasis. Proc Natl Acad Sci USA 1998; 95(13):7550-7555. 15. Anyanful A, Sakube Y, Takuwa K et al. The third and fourth tropomyosin isoforms of Caenorhabditis elegans are expressed in the pharynx and intestines and are essential for development and morphology.

46. Goodwin LO, Lees-Miller JP, Leonard MA et al. Four fibroblast tropomyosin isoforms are expressed from the rat alpha-tropomyosin gene via alternative RNA splicing and the use of two promoters. J Biol Chem 1991; 266(13):8408-8415. 26 Tropomyosin 47. Ruiz-Opazo N, Weinberger J, Nadal-Ginard B. Comparison of alpha-tropomyosin sequences from smooth and striated muscle. Nature 1985; 315(6014):67-70. 48. Helfman DM, Cheley S, Kuismanen E et al. Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

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