Culture of Marine Invertebrate Animals: Proceedings — 1st by John M. King (auth.), Walter L. Smith, Matoira H. Chanley

By John M. King (auth.), Walter L. Smith, Matoira H. Chanley (eds.)

This quantity is predicated on prec'entations on the convention on tradition of Marine Invertebrate Animals which used to be held in eco-friendly­ port, long island in October, 1972. The convention used to be backed through the center Atlantic typical Sciences Council, Inc., a non­ revenue academic company, including the Marine technological know-how facilities of Adelphi collage, the country college of recent York at Stony Brook, ny collage, Suffolk County neighborhood collage, and the guard Island Oyster corporation. the aim of the convention used to be to supply a wanted ex­ swap of data between scientists of assorted specialties whose info will be valuable to others faced with comparable difficulties, inspite of various marine animals. half I considers supportive ideas -- normal isolation and tradition tools, difficulties of sickness and feeding. particular concepts hired within the tradition of quite a lot of invertebrate organisms is roofed partially II. we wish to thank the members for his or her cooperation in getting ready the manuscripts in keeping with their convention shows. Walter L. Smith Matoira H. Chanley v Contents half I Recirculating procedure tradition tools for Marine Organisms .............•.

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Tris may be omitted. See Table 9. The vitamin solution used in other media given in this paper (Table 8) yields the concentrations required for this medium if used at 1 ml/l instead of 1/2 ml/l. The medium has proved satisfactory with 1/2 ml/l of vitamins. TABLE 11 Composition of Medium WC (see p. 05 1350 K Trace Elements: Per liter, use one ml of one of the trace element solutions described in Table 6 or 7 plus 1 ml of the boron stock solution given in Table 12. 25 rug B per liter). Check the pH if the more acid trace metal solutions are used and aqjust as necessary.

Y be omitted for organisms other than diatoms, The concentration used for diatom cultures may be adjusted according to the silicate concentration of the seawater used and the need for avoiding precipitation. Recently it has been found (by K,C. Haines and the author) that seawater enriched as "f/2" but without added Si will dissolve a great deal of silicate (up to 290 pM) from 125 ml borosilicate PHYTOPLANKTON FOR FEEDING MARINE INVERTEBRATES 49 glass flasks, on autoclaving. This does not occur in polycarbonate flasks, of course, and less will dissolve if the medium is autoclaved in large vessels.

Scrubbing with detergent is usually enough, but may leave some of the film of precipitate. In many laboratories the glassware is routinely treated momentarily with mild acid first, then dipped into detergent solution, rinsed with tap water, and finally rinsed with distilled or deionized water if the local tap water leaves a film. The mild acid is usually 20-30% commercial HCl, though H2S04 should do as well. A row of small flasks, emptied of contents, can be lined up and 60-75 ml of the acid poured into the first.

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